![neb noti hf neb noti hf](https://www.neb-online.de/wp-content/uploads/2015/04/Flexible_Enzyme_Gel_M.jpg)
![neb noti hf neb noti hf](http://neb.ebiomall.com/images/neb/r3138s_pdp_800x450.jpg)
Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.) Filing date Publication date Priority to US95920307P priority Critical Application filed by New England Biolabs Inc filed Critical New England Biolabs Inc Priority to EP08796186A priority patent/EP2167655B1/en Publication of EP2468854A1 publication Critical patent/EP2468854A1/en Application granted granted Critical Publication of EP2468854B1 publication Critical patent/EP2468854B1/en Status Active legal-status Critical Current Anticipated expiration legal-status Critical Links Original Assignee New England Biolabs Inc Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.) ( en Inventor Zhenyu Zhu Aine Blanchard Shuang-Yong Xu Shengxi Guan Hua Wei Penghua Zhang Dapeng Sun Siu-Hong Chan Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.) Granted Application number EP11075111A Other languages German ( de)
Neb noti hf pdf#
Google Patents High fidelity restriction endonucleasesÄownload PDF Info Publication number EP2468854A1 EP2468854A1 EP11075111A EP11075111A EP2468854A1 EP 2468854 A1 EP2468854 A1 EP 2468854A1 EP 11075111 A EP11075111 A EP 11075111A EP 11075111 A EP11075111 A EP 11075111A EP 2468854 A1 EP2468854 A1 EP 2468854A1 Authority EP European Patent Office Prior art keywords cleavage restriction endonuclease buffer mutation activity Prior art date Legal status (The legal status is an assumption and is not a legal conclusion. The items we carry in stock are listed in the table below as well as in the NEB UMaine Freezer Program website.To view the entire NEB catalog, visit their website here. Google Patents EP2468854A1 - High fidelity restriction endonucleases The purified PCR products was digested in a 30 l reaction consisting of 0.5 l of NotI HF (NEB), 0.5 l of PacI (NEB), 3 l of Cut Smart Buffer (NEB). The University of Maine Freezer Program receives a 15 discount on all products available in the New England Biolabs (NEB) catalog. PRODUCT NAME PRODUCT NUMBER BUFFER HF FACTOR AgeI-HF R3552 CutSmart 8 AgeI R0552 1.1 ApoI-HF R3566 CutSmart 25 ApoI R0566 3. et al (2008) Nucleic Acids Reseach 36, e50. EP2468854A1 - High fidelity restriction endonucleases HF ENZYMES 4 WHAT IS A HIGH-FIDELITY ENZYME Wild type enzymes were tested in supplied buffer for comparisons.